저알카리 생체활성유리의 생체 적합성에 관한 연구
Study on the Biocompatibility of Low Alkali Containing bioactive Glasses in Vitro and in Vivo
김영렬, 강선주, 박귀원, 김석화,
소속 상세정보
김영렬 ( ) - 서울대학교
강선주 ( ) - 서울대학교
박귀원 ( ) - 서울대학교
김석화 ( ) - 서울대학교
KMID : 0355819930170010015
Abstract
The purpose of this study was to evaluate the biocompatibility of low alkali containing bioactive glasses(55S Bioglass and 55SF4B-9.6N Bioglass) by means of in vitro cytotoxicity, subcutaneous and bone implant tests.
Using the culture of mouse fibroblast cells(L929, ATCC), cell growth and viability were analysed on 2nd, 4th and 6th experimental days. The succinate dehydrogenase(SDH) activity was examined by Millipore filter method, and Agar overlay method was
utilized to detect the acute cytotoxity of leachable components of glasses. Rectangular shaped(10mmx7mmx1mm) glasses were implanted in subcutaneous tissue of the back of guinea pigs. After 2 and 12 weeks implantation period, capsules surrounding
implanted materials were observed under light and electron microscope. For bone implant test, rectangular shaped(4mmx4mmx2mm) hydroxyaptite. 45S5 Bioglass, 52S Bioglass, 52S Bioglass, 52S Bioglass, 55Sf4b-9.6N Bioglass were implanted in the
drilled
defects on tibial diaphysis of rabbits. After 1 and 6 months of implantation period, bone reactions were observed under light microscope suing undecalcified group method.
@ES The results were as follows :
@EN 1. In both of 55S and 55SF4B=9.6N Bioglasses, the total number of fibroblasts was gradually increased for experimental periods and their survival rates were similar with those of negative control group after 2 and 4 days, and higher than
those
after 6 days. in the Millipore filter and Agar overlay tests, there were no cytotoxicities in both of 55S and 55SF4B-9.6N Bioglasses.
2. After 2 weeks subcutaneous implantation of 55S and 55SF4B 9.6N Bioglass, thick fibrous capsule with active fibroblasts were found around implanted materials and inflammatory cells in variable degree were observed at the superficial area of
capsule.
After 12 weeks, most of 55S Bioglass implants were encapsulated with thin noninflammatory fibrous capsule except a few implant capsules with moderate inflammatry cells. On the other hand, thin capsulc surrounding 55SF4B-9.6N Bioglass were
observed
with
groups of macrophages.
3. Insertion of 45S5 Bioglass, 52S bioglass and 55S Bioglass into a cortical defect drilled in tibia resulted in bonding with total bone within 1 monthl. On the other hand, partial soft tissue interface was observed around 55SF4B-9.6N Bioglass
up
to 6
months of implantation.
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